I'm working on the cell lines and want to investigate level of phosphoinositide PtdIns, PtdIns (3), PtdIns (5), PtdIns (3,5), PtdIns (3,4,5). Some paper said using the radiolabbeling but I would like to try to analyze without radiolabel, is it possibility?
I don't have experience in this experiment. If you have experience to analyze to measure phosphoinositide please let me know.
thank you
dewi