There are a few different coatings you can use for this:
I used poly-l-lysine, which worked very well and allowed the neutrophils to attach to the surface and also remain very motile. The drawback of using poly-l-lysine, is that neutrophils would not encounter this in-vivo and so it might not be suited for your experiments.
A more realistic representation of an in-vivo situation would involve using a coating such as gelatine or ICAM.
IF YOU COAT THE WELLS BY AGAROSE GEL YOU CAN SEE NEUTROPHİL MOVEMENTS UNDER AGAROSE. THE END OF STUDY YOU WİLL GET OUT AGAROSE GEL AND STAIN WELLS TO SEE MOVEMENT TRACES ON THE WELLS.
There is no need to coat your plate if you are activating your cells. They stick very strongly to plastic and glass bottom surface. Alternately, you may use Retronectin solution. http://www.clontech.com/US/Products/Viral_Transduction/Hematopoietic_Cell_Transduction/RetroNectin_Reagent