How to solve problem in over expression research?

Hi.. Recently I do over expression some gene using plasmid target and transfection in hek 293 cells. My plasmids are flag pcDNA3 and pCMV-myc plasmid. When I did that experiment then I analyze...

01 February 2017 4,175 2 View

How to measure phosphoinositide in cell line?

I'm working on the cell lines and want to investigate level of phosphoinositide PtdIns, PtdIns (3), PtdIns (5), PtdIns (3,5), PtdIns (3,4,5). Some paper said using the radiolabbeling but I would...

10 November 2016 2,521 1 View

How poly IC in transfection?

04 May 2016 9,342 2 View

Does anyone have experience in neutrophil movement?

i would like to investigate the movement of neutrophil in 96 well plate and i need to coat the well. does anyone have experience about the best coat for neutrophil movement? BSA? HSA? FBS?...

01 February 2016 8,306 4 View

Why do I have problems in transfection?

i check the condition of the cells. But I get the worst condition the cells. Only a few cells that attach on TCD. so, may you help me to solve electroporation problem? thanks

08 September 2015 3,636 6 View

What is the critical step when stimulation?

in my case, may I say that the production of IL10 of the supernatant isn't enough? Is it necessary to increase the cell number? Thanks for helping regards, Dewi

04 May 2015 9,408 1 View

Does anyone have some experience in PCR with gene length about 4,000bp?

01 February 2015 8,342 22 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View