8 Questions 24 Answers 0 Followers
Questions related from Dyaningtyas Dewi Pamungkas Putri
Hi.. Recently I do over expression some gene using plasmid target and transfection in hek 293 cells. My plasmids are flag pcDNA3 and pCMV-myc plasmid. When I did that experiment then I analyze...
02 February 2017 4,251 2 View
I'm working on the cell lines and want to investigate level of phosphoinositide PtdIns, PtdIns (3), PtdIns (5), PtdIns (3,5), PtdIns (3,4,5). Some paper said using the radiolabbeling but I would...
11 November 2016 2,599 1 View
Dear all, how microliter of DNA 1ug/ml I should apply in volume of dilution medium to get the final concentration is 1ug/ml in 96 well, 24 well and 6 well Thank you for your help
05 May 2016 9,421 2 View
i would like to investigate the movement of neutrophil in 96 well plate and i need to coat the well. does anyone have experience about the best coat for neutrophil movement? BSA? HSA? FBS?...
02 February 2016 8,382 4 View
Dear all. Do you know what is the critical point in electroporation and how to optimize that condition? Thanks
01 January 2016 1,900 2 View
i check the condition of the cells. But I get the worst condition the cells. Only a few cells that attach on TCD. so, may you help me to solve electroporation problem? thanks
09 September 2015 3,718 6 View
in my case, may I say that the production of IL10 of the supernatant isn't enough? Is it necessary to increase the cell number? Thanks for helping regards, Dewi
05 May 2015 9,490 1 View
I try to PCR my gene target, that length about 4,000 bp. First time PCR, I try with cDNA 5ng, each concentration primer 0.1mikroMolar, dNTP 0.4mM, KOD FX 1.0U. and I got the smear band, I try to...
02 February 2015 8,419 22 View
