What is the best method for soil sterilization?

I wanna know the proper temperature, humidity and duration of soil sterilization, and also possible chemicals applicable for soil sterilization. looking forward to hear from you colleagues.

11 December 2017 5,735 4 View

Dear colleagues, how can I get rid of formalin smell, that I've used for lab disinfection?

in fact, I have used formalin & KMnO4 for disinfecting a tissue culture lab & growth room, but the smell have not removed after 4 days, how can I get ride of it? any practical method to...

11 December 2017 842 10 View

Can plant absorb amino acids through foliar application?

I wanna know if foliar application of amino acid solutions is an appropriate method? if not, what is the best method to apply amino acids on plants?

09 October 2017 7,012 5 View

Is any one here familiar with application of Artificial Neural Network in plant science?

08 September 2017 219 4 View

How to find out the relative humidity of CuSO4.5H2O and KOH salts in a range of temperature between 5C to 50C?

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02 March 2017 8,161 4 View

If the RH and seed moisture content being provided, is it possible to calculate seed water potential?

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31 December 2016 5,619 9 View

Is it possible to generalize a hydrotermal model that obtained for a seed , to other seeds?

11 December 2016 9,720 1 View

Is it possible for mycorrhizal fungi to growth in callus tissue, which originated from root zone?

09 October 2016 5,754 9 View

How to measure intercellular pH of plant cells?

06 July 2016 6,355 8 View

No title

02 March 2021 3,060 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View