I want to know that if formation of cell wall reduces cell division rate?
and if cell division affect cell wall formation?
I wanna know the proper temperature, humidity and duration of soil sterilization, and also possible chemicals applicable for soil sterilization. looking forward to hear from you colleagues.
11 December 2017 5,896 4 View
in fact, I have used formalin & KMnO4 for disinfecting a tissue culture lab & growth room, but the smell have not removed after 4 days, how can I get ride of it? any practical method to...
11 December 2017 1,005 10 View
I wanna know if foliar application of amino acid solutions is an appropriate method? if not, what is the best method to apply amino acids on plants?
09 October 2017 7,145 5 View
I wanna assess the effect of some plant growth regulators and minerals using Artificial Neural Network, but I'm not familiar with the issue.... any guideline?
08 September 2017 406 4 View
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02 March 2017 8,298 4 View
31 December 2016 5,760 9 View
I'm trying to develop a hydrotermal model for seeds moisture content among various temperature. I wanna know that can this model be generalized for other non-experimented seeds?
11 December 2016 9,857 1 View
I want to know that is it possible to innoculate mycorryhza fungi in callus which initiated from root zone? or is it expected that mycorrihza fungi exhibit different reactions to calluses with...
09 October 2016 5,892 9 View
I'm looking for a simple method for measuring interstellar- cytoplasmic pH of plant cells.
06 July 2016 6,512 8 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View