Which software to use to statistically analyze Telomere Length_TRF_Southern Blot?

I am trying to measure telomere length in an ant species using the TRF method, which is a Southern blot technique. Currently, I am struggling with analyzing the images and would appreciate any...

29 January 2021 604 3 View

Are researchers less able to research with age?

In your opinion, how old is the researcher feeling that he is unable to absorb and retain information highly in light of the pressures of life and the huge technological development that makes us...

05 August 2019 3,429 68 View

What are the main reasons of the appearance of rarefactive solitary wave ?

I have used a model and i got a rarefactive solitary wave but i do not know if this is an advantage or disadvantage and what are the reasons of the appearance of such waves

12 April 2019 9,935 3 View

What is the difference between using KLH as carrier protein with peptides and KLH as antigen without peptides?

Hello, I am trying to inject KLH in chicken as antigen not conjugated with protein. is it correct to think that KLH is used as antigen? i know that KLH is well known for its potent and safe...

06 April 2019 4,265 4 View

When performing 0.7% agarose gel electrophorsis why there is no difference between a control DNA sample and that exposed to ionizing radiation?

07 November 2018 2,760 4 View

What is the differenc between lymphocyte subset can be detected by flow cytometry and MACS technique?

Lymphocyte subset

25 July 2018 5,762 6 View

What is the cause of failure of detection of significant quantitive trait loci ?

excuse me for that as recently joined this field and trying to make my way. I have a data set of 1545 SNP markers from NGS for many traits of 63 individuals (F2 intercross) the genotypes analyzed...

28 March 2018 7,913 4 View

Why dna not exit from the well of agarose gel

i have extract dna of bacteriophage and make gel electrophoresis and dna apper in the well only why not exit??

20 October 2016 2,754 5 View

Dose anyone have an article about sclerotium sp. metabolites?

Agriculture science

15 August 2013 765 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View