How to make two elements strike each other in ABAQUS?

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Signal peptide or fusion partners like thioredoxin for hydrophobic protein expression in E.Coli?

I am trying to express HIV1 antisense protein in E.Coli. The protein is hydrophobic and also has two N-terminal cysteine triplets. I am planning to add a pelb signal peptide for periplasmic...

31 January 2024 1,862 1 View

Can we add recombinant enterokinase to the column directly for on-column tag removal?

I am using cobalt column for purification. If anyone has done it, kindly share the protocol.

24 January 2024 2,928 0 View

What would be the approximate purified protein yield from HEK 293t cells?

Plasmid Concentration - 25ug per t75 flask Cell number - 10 million cells Protein size - 25 KDa Purification procedure - His tag purification using cobalt resin. I am going to use this purified...

15 November 2023 7,015 1 View

What can be the alternative of Acetic anhydride for acetylation of carbohydrates?

Since acetic anhydride is not available in our country, What can be the alternative of Acetic anhydride for acetylation of carbohydrates

13 December 2022 3,047 0 View

Which statiscal method is best to compare the cell viabilty percentages of two cell lines treated with same drug at different concentrations?

Im using wild type SW620 cancer cell line and shCD25 SW620 cells. I want to show the significant difference in the cell viability between both the cell lines to the chemotherapeutic drug...

24 August 2019 9,946 5 View

If I want to analysis the effect of combination of drugs do i need to select the IC50 values of both the drugs in MTT assay?

I want to analyse a particular protein cd24's role in chemo resistance, and I have found out with MTT assay that shCD24 cells shows more death than wild type cancer cells for 2 of the...

16 July 2019 8,916 3 View

How to functionalise Graphene with antibody?

Functionalising Graphene with antibody to carry out ELISA.. What are the various methods to link antibody?

24 August 2013 4,714 5 View

BIAM probe labeling and identification of labeling site

I am using a BIAM (N-(Biotinoyl)-N'-(Iodoacetyl)Ethylenediamine) probe to label reactive cysteine residues. I target to identify the labeling sites by LC MS/MS analysis. But the challenging part...

15 January 2013 8,754 2 View

How to get moment output in Abaqus Standart?

I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...

08 August 2024 4,831 4 View

How to fix errors in my heat transfer steel structure with reinforced concrete slab model Abaqus?

I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...

07 August 2024 1,028 0 View

Hello Everyone ! I'm looking for a good journal to publish my manuscript with low publication cost?

I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.

06 August 2024 6,796 3 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

Good day!! All, can anyone recommend the best battery related journals to download research articles please?

I am an Mphil research student and looking for high impact factor battery related journals to download and read research articles to improve my knowledge and get new ideas in the field of battery...

01 August 2024 6,516 2 View

About impact factor?

I have been publishing the Nepalese Journal of Agricultural Sciences (online ISSN 2091-0428; print ISSN 2091-042X) on www.nepjas.com and research gate. I was wondering how I can get an impact...

01 August 2024 1,277 1 View

Is the peer-reviewed publication "MedieKultur: Journal of Media and Communication Research" (E-ISSN 1901-9726, P-ISSN : 0900-9671) a legitima?

Is the peer-reviewed publication "MedieKultur: Journal of Media and Communication Research" (ISSN Online: 1901-9726, ISSN Print: 0900-9671) a legitimate and credible scholarly journal in the field...

01 August 2024 629 3 View

Simulation of metal drawing by Abaqus with UMAT?

Hello, colleagues. Recently, I have been working on a metal processing simulation with my UMAT in Abaqus. I have outlined the corresponding simulation, but I keep encountering issues that cause...

30 July 2024 7,062 1 View

Why running a restart analysis in Abaqus in Ubuntu OS gives an error as attached when running the same job in Windows doesn't give any error and runs?

I am trying to run a restart analysis, which imports deformed configurations of parts from a generated ODB file. It runs fine in Windows OS but when I try to run it in Linux OS, it is giving some...

29 July 2024 9,572 3 View

Majid Moghaddam

Hi Balakumaran Sivagnanasundaram

Have you defined contacts properly?

Martin Veidt

Balakumaran Sivagnanasundaram

You may probably not define the contact surfaces properly. I recommend you visit the below package to fully understand how to simulate impact analysis.

https://caeassistant.com/product/impact-analysis-in-abaqus/