I am using cobalt column for purification. If anyone has done it, kindly share the protocol.
I am trying to express HIV1 antisense protein in E.Coli. The protein is hydrophobic and also has two N-terminal cysteine triplets. I am planning to add a pelb signal peptide for periplasmic...
31 January 2024 1,862 1 View
Plasmid Concentration - 25ug per t75 flask Cell number - 10 million cells Protein size - 25 KDa Purification procedure - His tag purification using cobalt resin. I am going to use this purified...
15 November 2023 7,015 1 View
Since acetic anhydride is not available in our country, What can be the alternative of Acetic anhydride for acetylation of carbohydrates
13 December 2022 3,047 0 View
I have modelled a Beam and Car using Abaqus for impact analysis. During the analysis, the Car passes through (Penetrates) the beam without any impact. Can anyone please give me any valuable...
02 April 2022 6,676 2 View
Im using wild type SW620 cancer cell line and shCD25 SW620 cells. I want to show the significant difference in the cell viability between both the cell lines to the chemotherapeutic drug...
24 August 2019 9,946 5 View
I want to analyse a particular protein cd24's role in chemo resistance, and I have found out with MTT assay that shCD24 cells shows more death than wild type cancer cells for 2 of the...
16 July 2019 8,916 3 View
Functionalising Graphene with antibody to carry out ELISA.. What are the various methods to link antibody?
24 August 2013 4,714 5 View
I am using a BIAM (N-(Biotinoyl)-N'-(Iodoacetyl)Ethylenediamine) probe to label reactive cysteine residues. I target to identify the labeling sites by LC MS/MS analysis. But the challenging part...
15 January 2013 8,754 2 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...
06 August 2024 4,611 0 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...
30 July 2024 2,744 1 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View