hello, I need help to be able to make the separation of my two analytes that are nimesulide and 4-nitro-2-phenoxyaniline by HPLC, my column is cyano, the mobile phase is ACN / MeOH / Buffer pH 3.3 proportions 27: 3: 70
run analytes on tlc plates using different solvent mixtures at different proportions to establish good separation of analytes before using solvent mixtures at different proportions that gave good separation to run separate analytes on hplc.
Note: solvents that make up solvent mixtures to be used to run analytes on HPLC must be solvents permitted to be used on HPLC