HI,

I am working on vesicular stomatits virus ( VSV) and i should harvest high titer of VSV for about 500 ml. up to now i did TCID50 assay to measure the vsv titer i harvested and i got 10-5 . But i need 10-7 titer.

for inoculation of vero cell line by VSV, after three time washing the vero flask by PBS, i add 1 ml VSV and 5 ml DMEM to T25 flask of 80%-90% confluency for about 1 hour and after that put out the virus and finally i add DMEM with pen/strep and 1% FBS and incubate for 24 hour to see CPE.