How we choose the compound available for radiolabeling?

I think it's must contain an electrons donating group to be suitable for labeling by technetium 99m or iodine 125.

09 December 2018 7,795 2 View

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I tried to prepare chromium alum by ox-red reaction between K2Cr2O7 and ethanol in presence of H2SO4. The result is always a green liquid that dont crystallize. I have found that the reaction must...

24 November 2018 9,991 3 View

What is the best method for isolating and extracting PAHs from Leafy Vegetables?

Samples of leafy vegetables were taken. Trying to find a proper method to isolate PAHs content of vegetables. I would appreciate any help

28 July 2018 449 2 View

RTqPCR: How to evaluate the gene expression?

I did the RTqPCR of a gene in different cell lines samples and I used GAPDH as a housekeeping gene. I calculated the deltaCt and I want to calculate the fold change. What's the difference between...

05 June 2018 2,831 4 View

Databases for lung cancer ?

Hello, I used to work on gliomas and used several websites for RNA-seq such as Gliovis, geoprofile GDS1962 and many others to explore the mutation and the differential expression of genes in...

22 March 2018 5,739 4 View

What is paper or PDF of fredholm integro differential equation of first kind?

I need researchers of Fredholm integro differential equation of first kind

24 February 2016 5,287 3 View

Is there any possibility to contemporize Iranian Islamic Architecture In Globalization age?

31 January 2016 726 7 View

Is the bump in the beginnig of the diffraction patterns always an amorphous part?

I have always a bump in the diffraction patterns. The majority of peaks were indexed. The preparation temperature exceeded decomposition temperature of reagents and the melting point were not...

28 November 2014 2,720 9 View

Can anyone suggest a SAS procedure to analyze microRNA expression and a continuous variable (tumor volume)?

What SAS procedure can be used to analyze associations between microRNA (miRNA) expression profiles and a continuous variable (e.g., tumor size or certain scoring scale). I am trying to use SAS to...

25 June 2014 4,835 5 View

SEM model problem of unfitting model (e.g., RMR, RMSEA and chi-square)

Hello Can anyone help me with the poor fit model, the GFI,CFI, TLI and RMSEA which the most important as well as the Chi-square are performing low fit values, how to cure it?. Also, I realised...

01 January 1970 2,840 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Why the nucleic acid gel melted after running for 30min?

Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...

22 February 2021 9,896 3 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

How to dissolve lyophilized silk fibroin?

Dear members of the community, I am trying to concentrate silk fibroin to 25 % (w/v). Nevertheless, i am struggling with the concentration step. I successfully achieve concentrations of 5-6 % of...

16 February 2021 1,808 1 View