gel separator tube
Hi deaer I have complatede many sucessful mxene etching. Synthes method is delaminate 1g Ti3AlC2 MAX phase in 18.6 ml %37 HCI fume, 1.4 ml DI water and 1 g LiF. After sysntheisi I washed and...
16 October 2023 1,105 0 View
Hello, I am currently working on my research project in the field of additive manufacturing, specifically focusing on estimating the build time of FDM (Fused Deposition Modeling). As part of my...
10 May 2023 4,078 2 View
Dear All ; Would you please recommend a theory or a previous studies that can support my research. Your support is highly appreciated.
12 July 2022 7,432 4 View
I prepared hydrogel from extracellular matrix and measured the absorbance at 405 nm during 2 hours then blotting the points in excel to calculate speed of the gelation (s) , t lag and t50%. I...
08 June 2022 2,841 0 View
Is it membrane protein? Cell division/cycle cycle protein , transmembrane protein/ DNA inhibitor or something else?
28 January 2022 3,054 3 View
I used primers for a house keeping gene and gene of interest, the result showed in attached picture
27 November 2021 8,237 3 View
During RNA extraction I used preheated wash 1 solution( about 40C) "by mistake", discarded the filtrate, used wash2 and then the preheated elution buffer (the only buffer that should be heated...
08 November 2021 2,263 3 View
I used to use the plcg2 antibody (Q:20) from santa cruz for plcg2 detection in chicken . Unfortunately, it has been discontinued . Does anyone know of an antibody I can use instead (for Western...
11 October 2021 7,064 2 View
What is the meaning of having results when using a specific primer for E.coli gene, for example, in klebsiella pneumonia.. The gene product is present in both, but is it normal that the gene is...
02 August 2021 7,808 5 View
ESBL stands for extended spectrum beta-lactamase. It's an enzyme found in some strains of bacteria. ESBL-producing bacteria can't be killed by many of the antibiotics that doctors use to treat...
18 October 2020 4,369 4 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...
21 July 2024 5,128 4 View
Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...
19 July 2024 148 6 View
I have conducted molecular marker amplification experiment by PCR and performed PAGE with silver staining. Now i have to score all my gels for further genetic analysis. Manual gel reading is...
12 July 2024 7,145 2 View
