What is the meaning of having results when using a specific primer for E.coli gene, for example, in klebsiella pneumonia.. The gene product is present in both, but is it normal that the gene is typical in both?
Safa Naji
yes it is possible as they belong to the same family so try designing your primers on a housekeeping genes maybe that is not present in the other m.o
If the regions the primers anneal to are conserved between the genera then yes you would expect them to amplify the same region of DNA in all strains you test.
It is possible as long as you used in a very large range of nucleotides during designing since the conserved area would be quite long and possibly fit to different species of bacteria
You have two different questions.
Can PCR with specific primer of bacterial genus yeilds results using another bacterial genus?
If the primers are specific to the genes of bacteria of a certain species, they do not amplify the DNA of another species. Otherwise, they cannot be called specific.
What is the meaning of having results when using a specific primer for E.coli gene, for example, in klebsiella pneumonia.. The gene product is present in both, but is it normal that the gene is typical in both?
E.coli and klebsiella pneumoniae are closely related bacteria. It is not surprising that one pair of primers works on the DNA of both.
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