During RNA extraction I used preheated wash 1 solution( about 40C) "by mistake", discarded the filtrate, used wash2 and then the preheated elution buffer (the only buffer that should be heated according to instructions) ...
Does the heated wash solution make the RNA dissolve & go to the filtrate???
Note: we don't have the technics and materials for RNA electrophoresis
It's possible that some RNA was lost because of this, but probably not very much. The wash buffers contain around 75% ethanol, and they wash away salts (which will dissolve in 75% ethanol) but not RNA (which won't). Most substances are more soluble in any given solvent when the temperature is increased, but given that the solubility of RNA in 75% ethanol is really poor, I doubt it will have made much difference. Was the concentration of RNA around what you expected? If so, I don't think there's any reason to worry about the RNA quality or integrity.
Don't worry, You can carry on with your experiment, if you lost some of your RNA, it will not be much.
good luck
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