How to link a protein to a DNA oligonucleotide?

More Sergii Pochekailov's questions See All

Spermine detection by HPLC?

I tried to detect spermine using reverse phase HPLC and a derivatization agent, but a peak that I want to see on the chromatogramm is very low and is not significantly different from the base...

17 June 2019 5,515 3 View

3D model in Cupcarbon, is it possible?

Dear colleagues, I need to create WSN model in 3 dimensions. I tried to use Cupcarbon for it but I couldn't find this function there. Could you explain me, how to do it in the package? Or to...

08 November 2018 1,605 1 View

What are your opinions about perspectives of oxymoron like pathology diagnosis and treatment?

It’s known, that oxymorons are a proper subsets of the expressions, whose values mutually exclusive, and give us a semantic paradox. We proposed and tested algorithms for diagnosis of oxymoron...

12 October 2018 3,607 0 View

What are your opinions about perspectives of Moebius strip like pathology diagnosis and treatment?

Proposed and tested an algorithm for diagnosis of Moebius strip like pathology, as prerequisite for treatment correction. The algorithm is reduced to initialization of study objects as a...

12 October 2018 5,966 3 View

Where can I physically get a large library of protein genes?

I am studying massively parallel protein-protein interaction. Is there a good source for a ready DNA library, where a protein gene (or part of it) would be surrounded by standard adapters? Like...

05 July 2018 3,018 1 View

fluorescent voltage sensors use?

Dear friends, i have a question regarding the fluorescent voltage sensors. I never tried it before, so I need good advice. What is your experience with Voltage dyes? If would use the...

11 January 2018 5,622 2 View

Do you have a job opportunity in Blockchain area?

Dear colleagues, My current project will finish this December and I'm looking for a new job opportunity in a Blockchain researching area. Write me please if you have an open positions for...

17 December 2017 5,009 3 View

How can I calculate using of energy by WSN node?

Could you write a link where I can find formulas for calculation an energy which is used by node of WSN (for transfer a data)?

17 December 2017 7,814 14 View

What problems are there for implementation blockchain technology in IoT, WSN and mobile devices?

All know that there are a problem POW for small devices. What with what problems have you encountered when implementing blockchain for small devices? What criteria do you use for evaluate new...

12 December 2017 9,748 6 View

We are looking for blockchain researchers.?

Dear colleagues, We are looking for researchers who work with Blockchain technology for taking part in a program committee for Workshop on Blockchain Technologies and Security (BTS) of 9th...

10 December 2017 5,412 31 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

Gregory Poon

If your protein is Cys-free, you can clone in a spare Cys and couple it to a maleiamide-conjugated oligo. This will meet your criteria, although the coupling efficiency may be low.

Karo Michaelian

Tryptophan and tyrosine have a natural chemical affinity to their RNA anti-codons. You may want to try this for DNA. Let me know if it works.