After plating the neurons look okay but they all migrate and clump at the edge of the plate. How can I keep them at the center of the well? I am using the 6-well plate. I was told to place a drop of 0.5 ml of resuspended cells at the center, but last time I did so the drop got dispersed when I was moving the plate. I am worried that there may not be enough medium to cover all neurons.
Avoid shaking the well plates in circular motion as this will pull the cells away from the centre. To make it disperse, either you resuspend the cells gently, or just invert slightly.
I did not shake it at all. I actually did not want the drop to get dispersed. I want it to stay at the center of the plate well.
Naufal Kushairi
Hi,
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4438772/
This might help !!
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