Cheng-Ying Ho

9 Questions 13 Answers 0 Followers

Questions related from Cheng-Ying Ho

How to salvage underfixed tissue for immunofluorescence?

I have a bunch of precious samples that appear underfixed for immunofluorescence with a particular antibody. They are all in frozen OCT blocks now. Should I melt them down and re-fix them or can I...

12 January 2022 1,584 6 View

What is the best kit/method of RNA extraction for mouse DRG for RNA-seq?

I have tried Qiagen RNeasy Micro and Fibrous Tissue Mini. The yield is both low. I am wondering if there are better methods/techniques to increase the yield.

04 December 2019 9,441 3 View

How to keep DRG neuron cultures at the center of the plate?

After plating the neurons look okay but they all migrate and clump at the edge of the plate. How can I keep them at the center of the well? I am using the 6-well plate. I was told to place a drop...

16 August 2019 4,298 3 View

Any good BDNF antibody against human and mouse tissue for Western blot?

Since Santa Cruz discontinued their awesome rabbit antibodies a year ago, I have yet to find a replacement. Any recommendation on antibodies for Western blot? The antibody must recognize the...

21 January 2018 1,326 4 View

Gene expression profiling (gene chip) vs. RNA-seq

Need to conduct gene expression studies using cell lines (treated with a drug vs. no treatment). Is one method better than the other? Cost is a consideration too. Also want to know how many...

01 August 2017 2,165 4 View

For knock-in mutant mice, what is the difference between LSL and FLEx strategy? Is one better than the other?

I am using a company to make a knock-in mutant mouse. The company suggested the FLEx strategy (Cre on). How is it better than the traditional LSL strategy?

26 July 2017 409 2 View

What is the best method to analyze clonal architecture of cancer based on whole-exome or targe panel sequencing data?

I am looking into PhyloWGS. Can someone like me without bioinformatics background use it with ease?

14 February 2016 7,686 3 View

Any suggestions between Illumina or Ion Torrent platform for low-cellularity tumors?

I am struggling to get the next generation sequencing panel done for a few tumor cases: They are low-cellularity tumors. We only have scant FFPE tissue. The cases kept getting rejected by a...

31 December 2015 6,714 8 View

Paradoxical activation/inhibition of enzymes

I am trying to identify the substrates of a lysine deacetylase by proteomics. I treated the cells with a presumed activator of the protein deacetylase, lysed the cells and performed...

01 January 1970 3,827 4 View