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Questions related from Cheng-Ying Ho
I have a bunch of precious samples that appear underfixed for immunofluorescence with a particular antibody. They are all in frozen OCT blocks now. Should I melt them down and re-fix them or can I...
12 January 2022 1,540 6 View
I have tried Qiagen RNeasy Micro and Fibrous Tissue Mini. The yield is both low. I am wondering if there are better methods/techniques to increase the yield.
04 December 2019 9,383 3 View
After plating the neurons look okay but they all migrate and clump at the edge of the plate. How can I keep them at the center of the well? I am using the 6-well plate. I was told to place a drop...
16 August 2019 4,246 3 View
Since Santa Cruz discontinued their awesome rabbit antibodies a year ago, I have yet to find a replacement. Any recommendation on antibodies for Western blot? The antibody must recognize the...
21 January 2018 1,299 4 View
Need to conduct gene expression studies using cell lines (treated with a drug vs. no treatment). Is one method better than the other? Cost is a consideration too. Also want to know how many...
01 August 2017 2,125 4 View
I am using a company to make a knock-in mutant mouse. The company suggested the FLEx strategy (Cre on). How is it better than the traditional LSL strategy?
26 July 2017 382 2 View
I am looking into PhyloWGS. Can someone like me without bioinformatics background use it with ease?
14 February 2016 7,634 3 View
I am struggling to get the next generation sequencing panel done for a few tumor cases: They are low-cellularity tumors. We only have scant FFPE tissue. The cases kept getting rejected by a...
31 December 2015 6,672 8 View