I'm working on cancer model in mice. In my free-drug treatment group, there is slow growth of tumor as compared to control (Saline) group. But in the nano-particle treated Group, the tumor undergone apoptosis and complete disappearance of tumor was observed while the study plan was going on. So in this situation, how the study should be justified if in the paper, we will submit the histology only from the free drug treated Group and not the nano-particle treated Group ?
Should I re-replan the study only for this Nanoparticle treated Group and remove the tumor just before it spent off from the site of implantation so that I can perform histology of an apoptotic tissue atleast?
When providing a rationale for studying the total regression of a tumor, it is crucial to highlight the importance of histology in comprehending the underlying mechanisms and verifying the full regression. Here are few factors you might take into account:
1. Significance of histology: Histology offers intricate insights into the cellular and tissue structure, enabling a thorough evaluation of the tumor and its reaction to treatment. It aids in verifying the lack of remaining tumor cells and evaluating the degree of tumor regression.
2. Treatment response validation: Histological investigation can confirm the clinical response to treatment by verifying the absence of live tumor cells. It is essential to demonstrate the efficacy of the treatment regimen and ensure accurate assessment of treatment outcomes.
3. Mechanistic understanding of regression: Histology can offer valuable insights into the underlying mechanisms that drive tumor regression. This technique enables the evaluation of cellular and molecular alterations occurring in the tumor microenvironment, such as the infiltration of immune cells, the formation of new blood vessels, and programmed cell death. Gaining insight into these pathways can provide valuable guidance for future treatment approaches and contribute to the advancement of individualized medicines.
4. Documentation and reporting: Histological evaluation offers a uniform and unbiased assessment of tumor regression. It enables precise documenting and reporting of therapy response, allowing for comparison with other trials and aiding data analysis for future research.
To summarize, the justification for studying cases where a tumor completely regresses and the necessity of demonstrating histology involves highlighting the significance of histology in verifying the absence of any remaining tumor cells, validating the effectiveness of treatment, comprehending the mechanisms of regression, and guaranteeing precise documentation and reporting of treatment results.