Jagtar Singh

16 Questions 8 Answers 0 Followers

Questions related from Jagtar Singh

1H NMR spectra ?

Recently I performed 1H NMR spectroscopy of a compound, having 23 Hs. The spectral data is showing tnat it has 23 Hs. Is it due to minor instrumental error or something's wrong with the compound...

23 November 2024 2,003 2 View

How to justify your study when there is Complete regression of tumor and you have to show histology ?

I'm working on cancer model in mice. In my free-drug treatment group, there is slow growth of tumor as compared to control (Saline) group. But in the nano-particle treated Group, the tumor...

25 May 2024 5,880 1 View

Can antigen retrieval protocol work for IHC/IF for formalin fixed tissues fixed for more than 7 days or even 2-3 months ?

I want to ask if antigen retrieval process works for formalin fixed tissues of more than 7 days or even 2-3 months ? Or it should be done for tissues that are fixed for max upto 24 hours ?

06 May 2024 2,455 2 View

What is the way out when you are working on cancer cell lines and carcinogen induced in vivo cancer models and you have to perform Western blot?

I'm working on carcinogen induced colorectal cancer. I have induced cancer in mice, and the tumors have been induced in the colon as well as in the rectum which is visible externally. I'm also...

28 April 2024 939 3 View

Can Immunofluorescence and immunohistochemistry be performed on a tissue other than that that of used for H and E from same animal group ?

I am planning to do immunofluorescence (IF) and immunohistochemistry (IHC) from rodent tissues. Is it fine to perform IF and IHC from tissue sections from another tissue of same animal group that...

26 April 2024 521 2 View

Can we use FITC conjugated antibodies for more than 4 antibody Staining for immunofluorescence of same tissues ?

I have 5 primary antibodies for immunofluorescence (IF) Staining of tissues. For that, I have only FITC conjugated secondry antibodies. If I'll do IF for 5 different targets but all with same...

14 April 2024 9,027 2 View

Do antibodies diluted in P.B.S work ?

I've received antibodies diluted in P.B.S. instead of 5% BSA in 1X T.B.S. Will this dilution work for IHC or I've to discard the dilution ?

11 April 2024 6,883 1 View

Which Software is freely available for PK Modelling ?

I want to do mathematical modelling of Pharmacokinetic data from plasma drug concentration in mice. Can someone please suggest me any freely available software for PK Modelling.

23 March 2024 8,834 3 View

Targeting of drug encapsulated in nanoparticles

For most of the drugs, their target receptors are located on the cell membrane surface. While we encapsulate the drug inside the Nanoparticles, the drug-encapsulated Nanoparticle internalise in...

01 January 1970 1,321 1 View

Pharmacokinetic studies in mice

I am planning to conduct pharmacokinetic studies in mice. Considering the availability of low blood volumes per mice, how to collect the samples efficiently in time frames of 5 min, 15 min, 30...

01 January 1970 4,153 7 View

Tissue distribution/Biodistribution of drugs

Hi ! Can anyone guide me how to study the tissue distribution of drugs in rodents using MALDI-TOF spectroscopic imaging ?

01 January 1970 169 0 View

Development of Azoxymethane/Dextran sulfate sodium induced colorectal cancer in BALB/c mice

I want to know the observations of scholars who have developed Azoxymethane and Dextran sulphate sodium induced colorectal cancer in mice. There is a significant risk of mortality from...

01 January 1970 559 0 View

Tssue processing protocol

Can anyone share tissue processing protocol for mice brain ?

01 January 1970 1,127 2 View

Histology by SEM/TEM

Can anyone share the tissue processing protocol for SEM/TEM of tissue slides of mice?

01 January 1970 1,084 2 View

How to generate SMILE of a double stranded DNA molecule ?

If we know the base pair sequence of a double-stranded DNA, can we convert that sequence code into molecular formula, SMILE, and thus generating the structure of that molecule ? Can anyone suggest...

01 January 1970 108 0 View

Can 30 um thick tissue sections work for IHC, IF and TUNEL?

I have 30 um thick cryosections of rat tissue (eye ball). Can this much thickness work for IHC, IF and TUNEL assay ? What optimizations should be done to get as acceptable results as we get in 5-7...

01 January 1970 6,227 2 View