How to isolate mitochondria from blood ?

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Mourad Aribi

You can isolate mitochondria from activated blood cells, like LPS-activated monocytes/neutrophils.

Best

Rona R Ramsay

Isolate the cells first. Mitochondrial must be kept intact for isolation.

After blood cell isolation, mitochondria can be isolated easily by differential centrifugation.

but a gradient centrifugation gives cleaner mitochondria.

Pooja Dhakne

Thanks for your time to respond Mourad Aribi and Rona R Ramsay