You can isolate mitochondria from activated blood cells, like LPS-activated monocytes/neutrophils.

Best

Isolate the cells first. Mitochondrial must be kept intact for isolation.

After blood cell isolation, mitochondria can be isolated easily by differential centrifugation.

but a gradient centrifugation gives cleaner mitochondria.

Thanks for your time to respond Mourad Aribi and Rona R Ramsay