I'm trying to isolate DNA from fibroblast cultured cells without using a kit (with lysis buffer and prot K), but after PCR amplification, no products is shown on the agarose gel. However, the same DNA isolation method works in HEK293T cells.
Does anyone have experience with DNA isolation of fibroblast with lysis buffer or tips?
Thanks!
Md Zulfekar Ali
may read
https://www.thermofisher.com/bd/en/home/references/protocols/cell-culture/primary-cell-protocols/fibroblast-protocols.html
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Josephine Nguyen
Md Zulfekar Ali Thank you, however this is a protocol to isolate fibroblast. I meant DNA isolation of cultured fibroblasts cells.
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