I am searching for the protocol of Chromatophores isolation and culture from fish skin. Can anyone kindly guide me regarding the experiment?
Thanks in advance.
Timir Tripathi , certainly you are aware of the following article
- A Method for Extracting Pigments from Squid Doryteuthis pealeii by Christopher W. DiBona1, Thomas L. Williams1, Sean R. Dinneen1, Stephanie F. Jones Labadie1, Leila F. Deravi1,2 ,
1Department of Chemistry, University of New Hampshire,
2Materials Science Program, University of New Hampshire
Article A Method for Extracting Pigments from Squid Doryteuthis pealeii
Original 'web location': =
for the following 2 URL's: after pasting the link-address into your browser, delete first the underline character " _ " in between "http _ and s" before you click on "RETURN":
[ http_s://www.ncbi.nlm.nih.gov/pmc/articles/PMC5226083/ , for the original PDF:
http_s://www.ncbi.nlm.nih.gov/pmc/articles/PMC5226083/pdf/jove-117-54803.pdf] , in the pdf:
cf.: 2. Isolating Chromatophore Pigment Granules and
3. Pigment Extraction
and BTW, there exists also a Video of the technique(s):
https://www.jove.com/video/54803/a-method-for-extracting-pigments-from-squid-doryteuthis-pealeii
(NB: quote: "This is a sample clip. If you're new to JoVE sign up and start your free trial today to watch the full video! If your institution has an existing subscription, log in or sign up to access this video." End of quote.
There is an "old" source available (unfortunately only PPV, no warranty for "isolation techniques" contained) ):
Article 6 Chromatophores and Pigments
Fish Physiology
Volume 3, 1969, Pages 307-353
(NB: same as above = delete first the underline character " _ " in between "http _ and s" before you click on "RETURN": applies to:
http_s://www.sciencedirect.com/science/article/pii/S1546509808601168)
Article Purification of Black Moor Goldfish melanophores and respons...
In Vitro Cellular & Developmental Biology
- Purification of Black Moor Goldfish melanophores and responses to epinephrine, by Carl R. Clark, John D. Taylor, T. T. Tchen, June 1987, Volume 23, Issue 6, pp 417–421 (NB: same as above applies to: http_s://link.springer.com/article/10.1007/BF02623857)
- Cytophysiology of Fish Chromatophores by Ryozo Fujii
Article Cytophysiology of Fish Chromatophores
International Review of Cytology
Volume 143, 1993, Pages 191-216, 216a, 216b, 217-255
(NB: same as above applies to:
http_s://www.sciencedirect.com/science/article/abs/pii/S0074769608618768 )
- Chaplen FW, Upson RH, Mcfadden PN, Kolodziej W. in: Pigment Cell Res. 2002 Feb;15(1):19-26.
Fish chromatophores as cytosensors in a microscale device: detection of environmental toxins and bacterial pathogens.
Article Fish Chromatophores as Cytosensors in a Microscale Device: D...
(NB: same as above applies to:
http_s://www.ncbi.nlm.nih.gov/pubmed/11841070 )
- Skin Biopsies as Tools to Measure Fish Coloration and Colour Change
P. Andreas Svensson and Helen Nilsson Sköld 2011,
http://cdn.intechweb.org/pdfs/22593.pdf
Correct Citation: Andreas Svensson and Helen Nilsson Sköld (2011).
Skin Biopsies as Tools to Measure Fish Coloration and Colour Change, Skin Biopsy - Perspectives, Dr. Uday Khopkar (Ed.), ISBN: 978-953-307-290-6, InTech, Available from: http://www.intechopen.com/books/skin-biopsy-perspectives/skin-biopsies-as-tools-to-measure-fish-coloration-and-colour-chang
- From: Eberle A.N.: The Melanotropins. Chemistry, Physiology and Mechanisms of Action. Basel, Karger, 1988, pp 210-252 (DOI:10.1159/000415282)
11. Effects of MSH on Pigment Cells https://www.karger.com/Article/Pdf/415282
...confessing / believing, it might be personal 'fortune' to get presented a "comprehensive / universal' protocol for isolation of chromophores ....
Best of luck, WHM
Timir Tripathi Sir, I am interested to study the melanophores which containing Eumelanin or melanin basically.
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