Timir Tripathi

5 Questions 13 Answers 0 Followers

Questions related from Timir Tripathi

How can I study the metabolic effect of ethanol in a cell line?

I want to see the effect of ethanol on a particular bacteria. The effect of it on the cellular physiology, metabolic changes, gene expression etc. What techniques are available to study this problem.

25 June 2015 2,245 2 View

How to calculate kinetic parameters with NADPH coupled reactions?

Do we have to subtract or add the blank reading (which lacks enzyme), from the reading with protein? I am not able to understand the calculations. Please help me out.

28 February 2014 8,496 1 View

A protein of mine is showing structural stability from pH 2 to 11, can anyone explain this phenomenon?

Far UV CD, tryptophan fluorescence and SEC was used to confirm the results.

25 February 2014 5,142 3 View

Our protein is showing more molecular mass than expected in SEC (around 20% more).

Some papers say that this protein shows such behavior due to charge state distributions. I am not getting a proper explanation for the same. The protein is cytosolic, soluble when recombinantly...

15 March 2013 6,833 7 View

Purification problem with His-tag protein and Ni-NTA matrix

We are facing purification problem with a His-tag cloned gene. The lysis buffer is 50mM Phosphate pH 8.0, 300 mM NaCl and 1mM PMSF. The Protein (50 kda dimer) is bound to Ni-NTA Matrix and then...

03 January 2013 1,541 40 View