Hi all,
I have performed a pathway analysis on mirSystem with a bunch of miRNAs differentially regulated in my experiment. However, I have not been able to find much information on the score which the algorithm asigns to the hits. Can anyone help me with what would be a significant level? ( in my case, the top ranked pathway only had a score of 1.7).
Many thanks!
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0042390
According to the publication, score is the weighted sum of -log(p) values.
"If miRNA expression values were available, the ratios of experimental group to control group for queried miRNAs were incorporated into an additional weighted pathway-ranking method to identify the enriched biological functions. First, for a given set of miRNAs, expression levels of the miRNAs were required to be used as the weight. The weight for one miRNA was calculated by dividing its absolute expression value by the absolute sum of the expression values of all input miRNAs. Next, after identifying the target genes by the selected prediction algorithms, for each functional category, the ranking score was obtained by summation of the weight of its miRNA times its enrichment −log (P-value) from the predicted target genes"
- Badges
- Science topic
- Similar topics
- Biological Science
- Biochemistry
- Biomolecules
- Nucleic Acids
- RNA
- Small RNA
- microRNA