I am using IMR-32 cells as a neuroblastoma cell line model for MYCN amplified cells. This cell line has poor transfection efficiency. Using lipofectamine 3000 reagents, I am getting only about 30% efficiency for transfection. I am looking forward to the suggestions for increasing the transfection efficiency of IMR-32 to a higher-end.
As long as I remember, we always used electroporation for this same reason in IMR-32: it doesn't react well to Lipofectamine. Maybe you should try electroporation.
Hello, I have no feedback for this specific cell line and use of our Viromers (which are not lipid-based as Lipofectamine but polymer-based reagents). However, we have good data for other neuroblastoma cells, as well as glioblastoma cells.
Please, see attached publication list, on page 6.
It's worth trying our Viromer RED if it's for plasmid, or Viromer BLUE/GREEN for siRNA. You can contact our distributor DongIn Bio in South Korea, they will provide you with test samples if motivated. Would be nice to learn from you if it works with IMR-32 cells actually!
Best, Sandra
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