Amit Kumar Pradhan

9 Questions 8 Answers 0 Followers

Questions related from Amit Kumar Pradhan

How to improve RNA extraction using Trizol?

I have been extracting RNA using trizol method from plant tissues but sometimes during the extraction procedure there is low pellet formation by which the quality and quantity of RNA gets...

10 October 2018 9,216 0 View

How to increase the intensity of bands in sqRT Pcr analysis for gene expression?

I have tried increasing the no. of cycles as well as checked the annealing temp but still i get a faint bands wherein i m suppose to get bright ones. even it appears same in case of housekeeping...

10 October 2018 8,604 2 View

What might be the reason that during quality using 0.8% gel electrophoresis i got a single thick band but not two separate bands as its seen normaly?

I extracted RNA from the fresh tissues using RNA kit form GCC Biotech, normally during the Quality check using electrophoresis it showed Double band but today in my case i could get only one...

09 September 2018 3,680 4 View

Can someone suggest me what might be the possible reasons for the appearance of smear, faint and no bands in semiquantitative RT-PCR gene expression?

I use the primers synthesized by molbiogen and all the pcr components purchased freshly and cDNA synthesized shows expression in housekeeping gene. I tried in normal DNA i got faint bands for the...

08 August 2018 7,811 2 View

Does housekeeeping gene show two different band size when the cDNA is prepared using reverse primer instead of Random Hexamerase?

I extracted the RNA and prepared cDNA using the reverse primer of OsActin instead of Random hexamerase and did a PCR for the same, but to my surprise i found two bands of different size of two as...

08 August 2018 562 5 View

Is it possible to do acid digestion of plant material in big borosil testube using water bath optimizable to 100 degree celsius?

Acid digestion of rice roots in 70% conc. HNO3.

06 June 2018 855 2 View

Please can anyone suggest is it safe to keep RNA in -20 degree celsius for some days instead of -80?

i wish to store RNA after isolation temporarily in -20 degree celsius is that possible and how safe it is?

05 May 2018 4,890 3 View

Please Can anyone suggest me a method of powder sample preparation from rice root tissue for XRD analysis of presence of elements?

I wish to study the presence of elements in rice roots under heavymetal toxicity.

04 April 2018 1,760 2 View

Reasons for the use of CaCl2 solution in preparing Aluminium stress solution after growing in nutrient solution. why do we use CaCl2 solution?

02 February 2018 3,901 0 View