Is it possible to do acid digestion of plant material in big borosil testube using water bath optimizable to 100 degree celsius?

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How to improve RNA extraction using Trizol?

I have been extracting RNA using trizol method from plant tissues but sometimes during the extraction procedure there is low pellet formation by which the quality and quantity of RNA gets...

09 October 2018 9,101 0 View

How to increase the intensity of bands in sqRT Pcr analysis for gene expression?

I have tried increasing the no. of cycles as well as checked the annealing temp but still i get a faint bands wherein i m suppose to get bright ones. even it appears same in case of housekeeping...

09 October 2018 8,475 2 View

What might be the reason that during quality using 0.8% gel electrophoresis i got a single thick band but not two separate bands as its seen normaly?

I extracted RNA from the fresh tissues using RNA kit form GCC Biotech, normally during the Quality check using electrophoresis it showed Double band but today in my case i could get only one...

08 September 2018 3,595 4 View

Can someone suggest me what might be the possible reasons for the appearance of smear, faint and no bands in semiquantitative RT-PCR gene expression?

I use the primers synthesized by molbiogen and all the pcr components purchased freshly and cDNA synthesized shows expression in housekeeping gene. I tried in normal DNA i got faint bands for the...

07 August 2018 7,707 2 View

Does housekeeeping gene show two different band size when the cDNA is prepared using reverse primer instead of Random Hexamerase?

I extracted the RNA and prepared cDNA using the reverse primer of OsActin instead of Random hexamerase and did a PCR for the same, but to my surprise i found two bands of different size of two as...

07 August 2018 462 5 View

Please can anyone suggest is it safe to keep RNA in -20 degree celsius for some days instead of -80?

i wish to store RNA after isolation temporarily in -20 degree celsius is that possible and how safe it is?

04 May 2018 4,773 3 View

Please Can anyone suggest me a method of powder sample preparation from rice root tissue for XRD analysis of presence of elements?

I wish to study the presence of elements in rice roots under heavymetal toxicity.

03 April 2018 1,673 2 View

Reasons for the use of CaCl2 solution in preparing Aluminium stress solution after growing in nutrient solution. why do we use CaCl2 solution?

01 February 2018 3,764 0 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Necessary tube lenght to cool Natural gas in a dry-ice cooling bath?

I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...

27 February 2021 8,128 1 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

High aspect ratio hexahedral mesh optimization solver?

I am interested in evaluating mesh optimization solvers that converge to high aspect ratio unstructured hexahedral grids. In other words, I am interested in a solver that optimizes warpage,...

26 February 2021 7,279 2 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View