Reasons for the use of CaCl2 solution in preparing Aluminium stress solution after growing in nutrient solution. why do we use CaCl2 solution?

02 February 2018 0 4K Report

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How to improve RNA extraction using Trizol?

I have been extracting RNA using trizol method from plant tissues but sometimes during the extraction procedure there is low pellet formation by which the quality and quantity of RNA gets...

09 October 2018 9,255 0 View

How to increase the intensity of bands in sqRT Pcr analysis for gene expression?

I have tried increasing the no. of cycles as well as checked the annealing temp but still i get a faint bands wherein i m suppose to get bright ones. even it appears same in case of housekeeping...

09 October 2018 8,663 2 View

What might be the reason that during quality using 0.8% gel electrophoresis i got a single thick band but not two separate bands as its seen normaly?

I extracted RNA from the fresh tissues using RNA kit form GCC Biotech, normally during the Quality check using electrophoresis it showed Double band but today in my case i could get only one...

08 September 2018 3,723 4 View

Can someone suggest me what might be the possible reasons for the appearance of smear, faint and no bands in semiquantitative RT-PCR gene expression?

I use the primers synthesized by molbiogen and all the pcr components purchased freshly and cDNA synthesized shows expression in housekeeping gene. I tried in normal DNA i got faint bands for the...

07 August 2018 7,851 2 View

Does housekeeeping gene show two different band size when the cDNA is prepared using reverse primer instead of Random Hexamerase?

I extracted the RNA and prepared cDNA using the reverse primer of OsActin instead of Random hexamerase and did a PCR for the same, but to my surprise i found two bands of different size of two as...

07 August 2018 593 5 View

Is it possible to do acid digestion of plant material in big borosil testube using water bath optimizable to 100 degree celsius?

Acid digestion of rice roots in 70% conc. HNO3.

05 June 2018 901 2 View

Please can anyone suggest is it safe to keep RNA in -20 degree celsius for some days instead of -80?

i wish to store RNA after isolation temporarily in -20 degree celsius is that possible and how safe it is?

04 May 2018 4,925 3 View

Please Can anyone suggest me a method of powder sample preparation from rice root tissue for XRD analysis of presence of elements?

I wish to study the presence of elements in rice roots under heavymetal toxicity.

03 April 2018 1,803 2 View

What is the reason for current dropping in OER , LSV curve?

I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...

10 August 2024 3,629 1 View

How to enrich pig excreta for increasing nutrient quality organically ?

Pig slurry is rich in major and minor nutrients. Is there any way to improve / Enrich its manure quality to be used in agriculture organically ? please share your knowledge.

09 August 2024 5,605 2 View

What may be the reasons for failures of Tube toi Tube Sheet Joints in Boiler Drum ?

We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.

08 August 2024 3,161 0 View

Is there any cases algae not using the nutrient from the wastewater and grow normally?

I am working on microalgae cultivation using waste water. The initial concentration of nutrients were less but the microalgae has achieved biomass growth of 2 g/L. The final concentration of...

08 August 2024 4,812 2 View

How is matter moved through an ecosystem and movement of energy different from the movement of nutrients through ecosystems?

03 August 2024 4,859 0 View

Why does the flow of energy through the biosphere depend on the cycling of nutrients and matter and energy flow across the earth's subsystem?

03 August 2024 6,412 0 View

How is the flow of matter through an ecosystem different from the flow of energy matter and nutrients flow through an ecosystem?

03 August 2024 8,655 0 View

What is the role of oxidative stress in toxin-induced carcinogenesis?

What is the role of oxidative stress in toxin-induced carcinogenesis? I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from...

02 August 2024 3,174 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Could GSH level be more in CCL4 rats than negative control rats?

i compare between the antioxidant and oxidative stress level between negative control rats (saline) and CCL4 intoxicated rats. i found the level of GSH and TAC is more in toxic group than normal....

26 July 2024 1,887 0 View