Dear All, can anyone help me to increase the concentration of my enzyme without using cut-off membrane because this membrane affects on the purity of enzyme. Could anyone suggest better option than this method?
Hi there,
Maybe ammonium sulphate precipitation will do.
Dominique Liger thank you very much Sir for your suggestion, could you please explain more might be it will be helpful for me. thanks
Hi again, read the link below for more info:
https://en.wikipedia.org/wiki/Ammonium_sulfate_precipitation
Dominique Liger Sir, Thank you very much.
I am using one enzyme to find out the specific activity of different substrates at different wavelengths. Is this right? or should I need to use same wavelength for all substrates for a single enzyme?
01 February 2020 1,848 4 View
I am confused about how is it possible that the same enzyme has a different molecular weight from different bacteria, even the enzyme is same either from E.coli or Bacillus sp. one is giving 75...
09 October 2019 7,308 4 View
Dear all, I m extracting mono sugars from polysaccharides. I have already hydrolyzed my polysaccharide with acid treatments, now would you recommend me any method or formula with instrument to...
08 September 2019 4,434 2 View
What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help
08 May 2021 0 0 View
28 February 2021 2,534 2 View
Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...
23 February 2021 6,266 3 View
I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...
21 February 2021 5,379 2 View
Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...
21 February 2021 7,447 2 View
Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...
14 February 2021 2,610 1 View
Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...
12 February 2021 1,386 3 View
Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...
08 February 2021 7,285 1 View
I am trying to prepare a Polyvinyl alcohol membrane for carbondioxide adsorption
08 February 2021 7,493 3 View