I am confused about how is it possible that the same enzyme has a different molecular weight from different bacteria, even the enzyme is same either from E.coli or Bacillus sp. one is giving 75 kDa while the other is 91 kDa.
Hi there,
Of course MW can vary greatly for homologous enzymes from different species as the common gene ancestor hasn't evolved the same way in these different organisms.
On the opposite the same activity may be beard by two different proteins (ie. not originating from the same gene ancestor) which have evolved in a convergent way to catalyse the same reaction but with different architectures/strategies.
So the question is : what is the level of homology for these 2 proteins? Do they exhibit conserved sequences/domains?
Dominique Liger Sir, thank you very much for informative response, They have very less amount of conserved sequences having 21% identity. My enzyme showing 77 kDa Mw while the others have 97, 85 and 33 kDa, so i was confused how this is possible same enzyme but different Mw.
Adam B Shapiro Sir, I determined by SDS-PAGE and it was similar to the theoretical values of the same enzyme.
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