We want to run 10x Genomic Visium spatial transcriptomics on lung tissue from COVID-19+ patients. How can we inactivate the virus so it's safe to work with the tissue at BSL2? The first step is to freeze the tissue in a isopentane/liquid nitrogen bath. After putting the tissue on the slide it is incubated in 100% methanol for 30 minutes at -20 degrees. Would either of these steps inactivate the virus?
The Rise of New Coronavirus Infection (COVID-19): A Recent Update
- DOI: 10.14744/ejmo.2020.22222
Freezing doesn't kill it. The fixatives do. To make double sure, you could use a heat inactivation step. As little as 5 min @ 70C is supposedly sufficient. You could increase that a bit to allow for heat transfer into a chunk of tissue.
https://www.thelancet.com/journals/lanmic/article/PIIS2666-5247(20)30003-3/fulltext
Article On Facing the SARS-CoV-2 (COVID-19) with Combination of Nano...
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