Hello,
I'm using the Nuclear Extract Kit Active Motif to do TransAM NFkB assay kit after
I'm working with non adherent cells : B cells, more specifically mantle cell lymphoma cells
I have a cytoplasmic contamination (verified with Tubulin) in my nuclear extract so i need to improve the protocol
I already try to use a dounce homogenizer but the nuclear extract are again contaminated with cytoplasmic
Did someone use this kit with this problem or this type of cell and can help me ?
Thank you !
The Nuclear Extract Kit isolates high-quality nuclear, cytoplasmic or whole-cell extract from mammalian cell or tissue samples. This eliminates the need for optimising reagents and protocols, and ensures high yields every time. More importantly, standardising your extraction procedure with our high-quality reagents ensures your reproducible results
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