03 December 2020 1 2K Report

Have anyone tried extracting dna from around 10^6 cells using thermofisher’s TRIzol kit? During the last step, the protocol suggested using 300ul 8mM NaOH to dissolve DNA and isolate DNA from other insoluble impurities. The reason that protocol provides is that extracted DNA doesn’t dissolve well in water or Tris buffer. Is there a way to dissolve DNA efficiently using only 50ul of solvent and also isolate the impurities? The details of the protocol is provided.

More Yuhao Wan's questions See All
Similar questions and discussions