I want to to do sequential fractionation of my plant’s chloroform extract using separating funnel. In which solvent should I dissolve my extract as my extract is immiscible in water.
Try with methylene chloride or ethyl acetate.
Ethyly acetate is miscible with nhexane and chloroform, if i follow the series n-hexane, Ethyl acetate, chloroform, butanol and methanol.
The general procedure is to dissolve extract in water and then start the series but unfortunately my chloroform extract is immiscible in water.
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