How to make fractions of chloroform extract of plant, using seperating funnel?

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Faiq H S Hussain

Try with methylene chloride or ethyl acetate.

Fariha Imtiaz

Ethyly acetate is miscible with nhexane and chloroform, if i follow the series n-hexane, Ethyl acetate, chloroform, butanol and methanol.

The general procedure is to dissolve extract in water and then start the series but unfortunately my chloroform extract is immiscible in water.