Yuhao Wan

5 Questions 4 Answers 0 Followers

Questions related from Yuhao Wan

Seeding density for fibroblast?

I am super confused right now about the seeding density for fibroblast. I searched online and most of the company protocol said 4000/cm2. However, based on my own experience, that's clearly not...

24 February 2022 4,947 4 View

What are these weird things from cell culture?

Hi, anyone know what the heck are those square things in my ultracentrifuged medium?

23 February 2022 4,343 5 View

How to improve DNA extraction using TRIzol and chloroform?

Have anyone tried extracting dna from around 10^6 cells using thermofisher’s TRIzol kit? During the last step, the protocol suggested using 300ul 8mM NaOH to dissolve DNA and isolate DNA from...

03 December 2020 2,062 1 View

Does the concentration of the fluorescent compound affect the fluorescent reading on a 96-well plate?

I am trying to detect the fluorescent level of the extracted RNA sample on a 96-well plate. After extraction, I dilute the RNA sample in 20ul solvent. Can I load the 20ul RNA sample directly to...

01 December 2020 2,747 2 View

I am running into troubles for my extracellular vesicles pull down assay. Could anyone give me some suggestions?

I want to study heparin-binding proteins on the surface of exosomes, and I am using streptavidin dynabeads MyOne C1 conjugated with biotinylated heparin as the complex to pull down exosomes...

01 January 1970 1,769 0 View