Is it possible to perform cardiac puncture followed by PBS perfusion with a pump?

Hello all, I will be perfusing mice with PBS for brain harvest and a colleague would like to collect the blood via cardiac puncture (these are 18m old mice so would be nice to maximize use). Has...

15 February 2021 5,312 2 View

What is the difference between blocking buffer tbs vs. pbs and which should I use?

Hi everybody! I am doing western blots on bovine mammary epithelial cells to determine casein. We have two types of blocking buffer in the lab. PBS:...

01 February 2021 9,278 5 View

What types of tubes should I use for allantoin assay?

Hi everyone, I am doing an allantoin assay in urine and all the protocols seem pretty vague. I am currently using this website which seems to be the most helpful:...

07 January 2021 8,023 3 View

What is growing inside a frozen 10X Ripa buffer?

Hello all! I have a strange question today...I noticed that a few of my aliquotted 10x RIPA buffers that were in the -20 C had some kind of potential mold (white balls, some clusters, some with...

20 December 2020 7,424 3 View

Prediction of novel precursor miRNAs in viral genomes?

Dear, I performed a multi-sequence alignment (MSA) with all the complete genome sequences of SARS-related viruses. I then used RNALalifold from the ViennaRNA package, with default parameters, to...

06 December 2020 1,704 3 View

What are these called?

Upon tissue plating for cell isolation, I start to see fibroblasts and after a while these 3D structures start to form. Are they called stroma? Someone in my building told me that she calls them...

30 November 2020 8,685 4 View

Plated Secretory Tissue from Udder--What type of cells are these?

Hello all! I plated some secretory tissue from the udder of a dairy cow. I am trying to isolate mammary epithelial cells. I found these cells on one of my plates, are these developing mammary...

28 October 2020 7,768 3 View

How difficult is Lysosensor to use in the lab?

How difficult is Lysosensor to use in a basic cell culture wet lab? Does anybody happen to have any advice and/or experience with this or similar probes? Thanks in advance!

15 September 2020 8,730 7 View

Quand est ce qu'un chercheur peut-il se sentir satisfait de ses publications?

18 May 2020 7,762 2 View

No title

18 May 2020 6,271 2 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View