Hi, I am separating triglycerides from oils using HPLC. I have run a standard solution containing 5 standard triglycerides. I found that retention time was based on their carbon no.
Later I separated triglycerides of oil samples. I found multiple peaks. Now I am confused to which group do they belong (LLL, LOP, MOP, LnLO etc etc etc). The literature says that use effective carbon no. method. ECN = CN-2DB, where DB= no. of double bonds.
Now to identify triglycerides in my sample should I plot a graph of Retention Time of Std triglycerides Vs their ECN and based on the should I check the ECN of sample triglycerides, thereby identifying the triglyceride composition?
attached is the standard protcol.
Some companies i think they sale yet pure tryglicerides standards. However an easyest way is taking pure oil sample, they have very characteristic trygliceride fingerprint that allows you to identify at the least the main peaks (as you show in the annex for soya oil). You can also use relative retention times (RRT) relatively to, for example OOO , which is is the main trygliceride in olive oil, regards.
Thank you so much for your reply. I will try finding a pure oil!
Could be useful read : ANNEX XVIII DETERMINATION OF THE DIFFERENCE BETWEEN ACTUAL AND THEORETICAL CONTENT OF TRIACYLGLYCEROLS WITH ECN 42. Commission Regulation (EEC) No 2568/91 of 11 July 1991.
Look at page 65 of the EU Regulation 1348/2013, Fig.1.
You can download that Regulation at the link below (3rd item).
Regulation 2568/91 has been changed more than once since its first release in 1991 and, on the TG analysis, 1348/2013 is the most up-to-date one.
Best,
Mauro
http://eur-lex.europa.eu/search.html?DTN=1348&DTA=2013&qid=1425895774094&DB_TYPE_OF_ACT=regulation&CASE_LAW_SUMMARY=false&DTS_DOM=ALL&typeOfActStatus=REGULATION&type=advanced&lang=en&SUBDOM_INIT=ALL_ALL&DTS_SUBDOM=ALL_ALL
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