Mahesha M. Poojary

12 Questions 51 Answers 0 Followers

Questions related from Mahesha M. Poojary

Effect of different buffers with the same pH?

How do they effect enzymatic reactions? say phosphate and citrate buffers at pH 6.

01 January 2016 2,048 1 View

How to perform Grouping of Pairwise comparison obtained after Tukey Test/One way ANOVA?

I am using Origin 9.1 software for one way ANOVA of my data. The data is attached, I want to compare the mean using Tukey test and represent the significant difference among the means (of control,...

01 January 2016 3,773 5 View

In vitro antioxidant assays-waste of money and time?

Dear All, Recently, I have attended two conferences in Europe (EuroFedLipid, Florence, Italy and EuroFoodChem, Madrid, Spain). During many presentations on ANTIOXIDANTS, I have observed that, many...

10 October 2015 7,832 94 View

Methods for amino acid extraction from plant sources?

Please share your ideas on possible methods of free amino acid extraction. Normally we use dil HCl for extraction. I wish to extract from other medium. Can anyone suggest what are the other...

08 August 2015 2,313 10 View

How to identify triglycerides in HPLC chromatogram?

Hi, I am separating triglycerides from oils using HPLC. I have run a standard solution containing 5 standard triglycerides. I found that retention time was based on their carbon no. Later I...

03 March 2015 4,779 5 View

WHy do I get High back pressure in my HPLC system after the column?

I am using Agilent 1200 HPLC-DAD system. When the detector end of the column is not connected to the detector line, the regular pressure in my HPLC is 53 bar at 1 ml/min flow rate (with column but...

03 March 2015 2,541 18 View

The difference in the productivity and selectivity of phyochemical extraction in laboratory scale compared to the same in industrial scale?

We have optimized the extraction some phytochemicals in laboratory scale.How can I contend that our optimization should work at a larger scale? What are the factors or aspects influence the...

03 March 2015 8,963 9 View

Can anyone provide me JCPDS XRD files for Ag and Au Nanoparticles?

I need JCPDS file for Ag and Au Nanoparticles (NPs were synthesized using biomaterials). Literature says that for Ag NPs, JCPDS file no. is JCPDS file no. 04-0783, while for Au NPs it is...

01 January 2015 9,942 18 View

Is there any problem in analysing triglycerides in oils by HPLC-DAD?

I found several articles with detectors being used ELSD or Refractive Index. I have DAD in my lab. Can we use it for analysis of triglycerides in vegetable oils?

01 January 2015 6,691 6 View

Low R-squared values in multiple regression analysis is allowed to publish in journals?

Hi, I am working on response surface models, using multiple regression equations to fit the experimental data. I am getting R2 values between 0.777-0.888. Lack of fit was found to be...

10 October 2014 5,081 2 View

Does anyone know how to plot 3D surface graph?

I have data that looks like this: X -- Y -- Z 25--30 --1.817 25-- 40 -- 1.763 25-- 50 -- 1.716 50-- 30 --1.748 50-- 40 --1.659 50 --50 --1.648 50 --30 --1.654 50 --40 --1.563 50 --50 --1.561 I...

04 April 2014 3,708 18 View

Injector and detector nuts are getting unscrewed automatically!!?

I am analyzing some sample using a GC keeping oven, injector, FID temperatures about 350 oC, 370 oC and 380 oC respectively. Unfortunately, after each analysis, when the column oven reaches...

03 March 2014 4,551 1 View