How to identify the speciffic plasmids containing ARGs during plasmid DNA extraction?

Chandrika Priyadarshani @Chandrika-Priyadarshani

30 August 2024 1 9K Report

I need to identify the plasmids carrying ARGs and to sequence them. How can I recognize the exact plasmid harboring the concerned ARGs

Sudarshan Murthy

Hello Chandrika Priyadarshani ,

Isolate the plasmid - Transform to competent cell and grow them in agar plate with antibiotic. The colonies which grow are resistant to that antibiotic.

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