Any easy method to find custom peptide concentration?
We have some synthesized peptides for our biotechnology and biochemistry based research, i am looking to find it's concentration.
Anybody can suggest best methods to find it?
Thanks
This question is not trivial. I assume that you do not have highly purified peptides as reference compounds. Usually, amino acid analysis is performed to quantify peptides. Often companies, which perform custom peptide synthesis, also offer a quantification by routine amino acid analysis. You may also consider a simplified amino acid analysis without derivatization, only based on tyrosine or phenylalanine, if your peptides contain one of these.
https://www.hindawi.com/journals/jaa/2016/7374316/
This question is not trivial. I assume that you do not have highly purified peptides as reference compounds. Usually, amino acid analysis is performed to quantify peptides. Often companies, which perform custom peptide synthesis, also offer a quantification by routine amino acid analysis. You may also consider a simplified amino acid analysis without derivatization, only based on tyrosine or phenylalanine, if your peptides contain one of these.
https://www.hindawi.com/journals/jaa/2016/7374316/
Determining peptide concentrations accurately and quickly has proven difficult for many researchers. Most commonly used methods for peptide quantitation rely on the weight of the lyophilized powder, absorbance of ultraviolet (UV) light or amino acid analysis. https://www.emdmillipore.com/Web-PR-Site/en_CA/-/USD/ShowDocument-Pronet?id=201306.9910
Also check https://www.biorxiv.org/content/biorxiv/early/2019/07/16/703397.full.pdf
Please look at the following below links which may help you in your analysis:
https://www.biorxiv.org/content/biorxiv/early/2019/07/16/703397.full.pdf
Thanks
Dear Mariyam K Kurshi
According to the article, “Fast and accurate peptide quantitation using the Direct Detect® spectrometer” the Direct Detect® spectrometer provides a universal, fast and accurate peptide quantitation method that does not require sample manipulation.
Please find the document via the link:
https://www.emdmillipore.com/Web-PR-Site/en_CA/-/USD/ShowDocument-Pronet?id=201306.9910
Hi Saraniya Bharathi
Dissolve the peptide in to water or any solvent
Use it in UV-Visible Spectrometer and check UV absorption of your peptide.
So you can found its concentration.
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