How to identify all the interacting proteins of a 100nt long rna , can ms2 tagged affinity purification be used ?

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Which satellite image gives best results for Land Use land cover classification(specifically for built up regions) for Indian region ?

Landsat, sentinel and USGS satellite images have varying accuracy for identification of urban built up dynamics ? Which one is best suited for India

04 February 2021 8,632 8 View

Can Cas13a protein directly purify from PAGE gel, please suggest me the method?

I am willing to Cas13 in vitro. Can it be directly purified from PAGE gel, please suggest me the method.

19 January 2021 1,136 3 View

Does behavior (shy vs bold; rare vs abundant) and flock size (small vs large) of bird species play role in niche partitioning among sympatric species?

Sympatric birds may avoid competition via multiple strategies - by different activity period, different diet, different microhabitat etc. Has any one looked into behavior of sympatric birds as...

11 January 2021 9,600 1 View

CPLEX Optimization tutorials ?

Respected all, Can anyone help me regarding where I can get complete and proper tutorials on IBM CPLEX Optimization software? I found some tutorials on Youtube...but they are just basic. I need...

05 January 2021 6,751 6 View

What is the correct formula to calculate NDWI ?

Gao (1996) gave the expression to calculate NDWI using Near Infrared (NIR) and Short wave Infrared (SIR), while some research suggests the use of the Green (G) band and Near Infrared (NIR) bands....

28 December 2020 9,938 2 View

How the problem of water scarcity in hilly region can be solved ?

the area received high rainfall, only last 3 months of hydrological year faces acute water shortage even for drinking the solution for this may be rainwater harvesting now how value...

25 December 2020 2,408 4 View

[SAP2000 related] I wonder why there is no any effect in the analysis result when I change the different parameters while modeling a frame structure?

(i) I have modeled a simple 2D frame structure (2 columns and 1 beam). I would like to see the variation in the result by varying the different parameters such as, sizes of confinement bars,...

06 October 2020 9,496 5 View

What is the most sensitive Non-invasive prenatal testing for fetal sex determination?

Is 8th week the earliest week where we can determine the fetal sex ?

05 October 2020 9,576 9 View

Global and local temporal structures in videos?

While learning action recognition we need to exploit both global and local temporal features. But cant understand the difference between the two??

03 August 2020 9,899 3 View

Some metal metal absorbs the most heat and some metal less. why?

Non-metallic materials such as brick stone and brick are good absorbers of solar energy, especially if they have dark coloring. Material with low thermal conductivity insulators they do not...

23 July 2020 5,503 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View