We plan to isolate proteins from microtome sections, but we do not understand under what conditions this can be done. What matrices for lysis, what volume of buffer, etc. Maybe someone has encountered such a problem?
what is carbon quantum dots anti bacterial characteristics in nano food packaging?
07 December 2020 5,008 3 View
Hello everyone. Do you know if it is possible to simulate a water filter using a porous medium in fluent? I would like to model a flow of water containing particles in a sand filter and see how...
21 January 2019 3,796 3 View
Has anyone encountered research literature surrounding the connection between emotion recognition or emotional intelligence and lineup identification or facial recognition?
23 May 2018 4,668 3 View
Dear Colleague, It would be your generosity to respond to the questionnaires and also distribute it among your colleagues, students, and networks. We would like to ask you if you would be so...
23 January 2018 7,141 12 View
so anybody have any idia how to convert this non solid material to solid constituents ,i mean if any gentleman done this work for any plants can explain it how to separate this extracted to solid...
14 December 2014 3,781 2 View
Is there a way I can see how many proteins are possibly in a SDS page band on a MALDI mass spec. Kind regards, Dara
30 November 2014 5,533 2 View
26 October 2014 2,308 6 View
01 January 1970 5,023 3 View
Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...
03 March 2021 1,499 2 View
Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...
03 March 2021 6,299 3 View
Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...
01 March 2021 2,215 2 View
I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...
01 March 2021 3,610 1 View
We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...
01 March 2021 4,937 1 View
Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.
01 March 2021 9,952 3 View
I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...
01 March 2021 9,015 2 View
Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...
01 March 2021 9,511 3 View
Let F be a field. Consider U, the set of n times n strictly upper triangular matrices in F. For X, Y in U, we call them similar if there exists some S, which is non-degenerate and upper...
01 March 2021 2,957 8 View
I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...
26 February 2021 7,129 3 View