We plan to isolate proteins from microtome sections, but we do not understand under what conditions this can be done. What matrices for lysis, what volume of buffer, etc. Maybe someone has encountered such a problem?
Depending on different journal articles and some with method with clear designs and some without method section and still called as journal articles ( can be derived a lot from reputed publisher),...
18 April 2024 4,713 5 View
Colleagues, good afternoon! We plan to conjugate antibodies with a fluorescent dyes. One source says that it is possible to conjugate anebodies that already have tag in the form of HRP, for...
20 March 2024 5,407 2 View
We want to compare the glycosylated and deglycosylated forms of the target protein on a blot. Tell me how to understand what form of glycosylation a particular protein has? I can’t find reagents...
01 February 2024 6,520 4 View
What are your thoughts?
26 August 2023 6,396 5 View
how can i extract a full list of my publications?
29 July 2023 9,438 3 View
I am trying to work out the activity of trypsin in pancreatin using the TAME assay. I am following the Minekus et al method, detailed in INFOGEST (as this is a precursor to an INFOGEST I am...
31 May 2022 5,859 1 View
Considering things like: time of administration, ease for researcher, cost (of course, though not the only factor), ease of revisiting the interview if consulting w/ team afterwards, etc. Thanks...
16 March 2021 8,010 0 View
what is carbon quantum dots anti bacterial characteristics in nano food packaging?
07 December 2020 5,284 3 View
Dear Sir or madam, I would like to understand what illnesses I am having. My symptoms are diarrhea with greazily floating stools and tightness on the left upper stomach with high heart rate.
25 May 2020 4,275 0 View
I am currently conducting a meta-analysis on the lipid profiles of mollusks fed different algae diets (with distinct lipid profiles). I am looking for any relevant articles or reports,...
05 February 2019 266 1 View
I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...
07 August 2024 1,028 0 View
I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...
07 August 2024 1,686 1 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,615 0 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,217 4 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,372 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,781 2 View
I want to Estimate surface heat fluxes using MyLake, but I don't have all the initial values in model parameters section and other sections,is there a way?
04 August 2024 1,535 1 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 966 3 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,122 4 View