Дарья Коломийцева

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Questions related from Дарья Коломийцева

How to homogenize microtome sections to extract protein?

We plan to isolate proteins from microtome sections, but we do not understand under what conditions this can be done. What matrices for lysis, what volume of buffer, etc. Maybe someone has...

28 March 2024 1,795 0 View

How to properly conjugate antibodies to fluorescent dyes?

Colleagues, good afternoon! We plan to conjugate antibodies with a fluorescent dyes. One source says that it is possible to conjugate anebodies that already have tag in the form of HRP, for...

21 March 2024 5,364 2 View

How to find out the type of protein glycosylation?

We want to compare the glycosylated and deglycosylated forms of the target protein on a blot. Tell me how to understand what form of glycosylation a particular protein has? I can’t find reagents...

02 February 2024 6,483 4 View