We have been trying to harvest the pericardial adipose tissue from mice, but we turned out to resect thymus after we send the specimen for microscopic analysis. Does anyone has experience in this procedure or can provide some online video if you find some?
I found one on JOVE. But, the video did not get into the details about pericardial fat. Only periaortic fat dissection is provided in detail.
Interesting.
Thank you for this question, that I 'll follow with great attention.
I have often found this problem, as an Anatomist with interest in the study of vasculature. When trying to canulate the aorta, through the left ventricle, to perform whole body injection of young adolescent Wistar rats, I usually first have to remove a certain amount of fat, that I presume to belong both to the thymus (more evident in young rats), but also to the pericardium. I never got to analyse it, but will be interested to know the opinion of other researchers.
Thank you.
Hi Ichun Chen, I am not sure about how to dissect pericardial fat, but I am interested in dissecting the periaortic fat. You have mentioned a JOVE video that I was not able to find. Would you mind of sending me the page link on JOVE where you have found it?
Best!
Hi
In this article (Figure 3) you may find helpful clues.
Effects of peroxisome proliferator-activated receptor δ on placentation, adiposity, and colorectal cancer.
and the Jove link for periaortic fat tissue is:
http://www.jove.com/video/54017/calcification-vascular-smooth-muscle-cells-imaging-aortic
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