Ichun Chen

10 Questions 5 Answers 0 Followers

Questions related from Ichun Chen

Neoantigen prediction in mouse model?

Currently, there are many methods or algorithms to predict neoantigen in human samples. However, if we would like to predict neoantigen from murine cancer cell lines in C57BL6/J background, does...

22 February 2018 828 1 View

How to analyze CFSE or Celltrace Violte dilution in Antigen presentation assay by flow cytometry?

Dear all, I am working on an antigen presentation assay now. The cells do proliferate well after 3 days of culture, esp. when we observed them under microscopy. We can see several cell clumps and...

25 July 2017 3,668 4 View

Are there any Dendritic cell specific organelle under Transmission Electron Microscopy?

Dear all, I am new to TEM that I would like to know if there's any dendritic cell specific organelle under TEM observation? Birbeck body/ granule seems to be specific only for skin...

06 February 2017 1,102 1 View

Murine breast cancer cell lines with bone metastasis potential?

Dear all, Does anyone know if E0771 from C57BL/6 can metastasize to bone regularly? Or, are there other murine breast cancer cell lines from C57BL/6 background that give rise to bone...

11 February 2016 8,304 2 View

How to estimate the amount of RIPA buffer needed in each plate for protein extraction?

I am using RAW cells in recent experiment now. However, in previous experiment, when I used 6-well plate for treatment (adding 80ul RIPA buffer into each well), the protein concentration was too...

12 April 2015 2,322 4 View

How to harvest the pericardial fat tissue?

We have been trying to harvest the pericardial adipose tissue from mice, but we turned out to resect thymus after we send the specimen for microscopic analysis. Does anyone has experience in this...

27 January 2015 5,179 3 View

Does anyone have knowledge on 27-hydroxycholesterol levels in tissue?

Are there anyone familiar with the measurement method of 27-hydroxycholesterol in peripheral tissues, such as subcutaneous fat or liver? I can not find methods for this oxycholesterol measurement.

14 August 2014 1,752 5 View

Can someone advise on murine estrogen receptor positive cell line?

I am looking for murine ER positive cell line now. However, from ATCC website, Estrogen receptor status was not reported. While checking other publication, C4HD cell line was found. But, C4HD is...

29 April 2014 4,783 3 View

Does anyone have experience using an Estrogen supplement in drinking water for a MCF7 xenograft?

We are working ona MCF7 xenograft model and will try to supplement estrogen by either E2 pellet implantation or E2 in drinking water. However, most of the papers did not mention the timing that...

02 February 2014 7,832 2 View

What kind of estrogen supplement should I use for my MCF7 tumorigenicity in nude mice model?

We are working on MCF7 tumor model on Balb/c AnN.Cg-Foxn1nu/CrlNarl nude mice now. Concerning the estrogen supplement, are there methods other than daily subcutaneous injection that work well? If...

13 January 2014 2,303 6 View