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Questions related from Ichun Chen
Currently, there are many methods or algorithms to predict neoantigen in human samples. However, if we would like to predict neoantigen from murine cancer cell lines in C57BL6/J background, does...
22 February 2018 775 1 View
Dear all, I am working on an antigen presentation assay now. The cells do proliferate well after 3 days of culture, esp. when we observed them under microscopy. We can see several cell clumps and...
25 July 2017 3,618 4 View
Dear all, I am new to TEM that I would like to know if there's any dendritic cell specific organelle under TEM observation? Birbeck body/ granule seems to be specific only for skin...
06 February 2017 1,028 1 View
Dear all, Does anyone know if E0771 from C57BL/6 can metastasize to bone regularly? Or, are there other murine breast cancer cell lines from C57BL/6 background that give rise to bone...
11 February 2016 8,253 2 View
I am using RAW cells in recent experiment now. However, in previous experiment, when I used 6-well plate for treatment (adding 80ul RIPA buffer into each well), the protein concentration was too...
12 April 2015 2,273 4 View
We have been trying to harvest the pericardial adipose tissue from mice, but we turned out to resect thymus after we send the specimen for microscopic analysis. Does anyone has experience in this...
27 January 2015 5,130 3 View
Are there anyone familiar with the measurement method of 27-hydroxycholesterol in peripheral tissues, such as subcutaneous fat or liver? I can not find methods for this oxycholesterol measurement.
14 August 2014 1,710 5 View
I am looking for murine ER positive cell line now. However, from ATCC website, Estrogen receptor status was not reported. While checking other publication, C4HD cell line was found. But, C4HD is...
29 April 2014 4,730 3 View
We are working ona MCF7 xenograft model and will try to supplement estrogen by either E2 pellet implantation or E2 in drinking water. However, most of the papers did not mention the timing that...
02 February 2014 7,784 2 View