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Questions related from Elizabeth Radley
Is it possible to grow a single dish of adherent mammalian cells (e.g. HEK293) where the cells form a continuous layer but there are different media compartments? We are looking at cell:cell...
04 August 2023 2,753 1 View
We are attempting to differentiate hiPSCs to cortical neurons and will be growing them on glass coverslips. We have to triple coat the glass with poly-L-ornithine (PLO), laminin and fibronectin....
14 February 2023 8,649 0 View
My lab is starting a new line of iPSC culture (KOLF2.1j). The cell suppliers provide a maintenance culture protocol using StemFlex medium with RevitaCell Supplement (both Gibco) on Synthemax-II-SC...
25 October 2022 2,714 3 View
My lab has been having issues re-amplifying a M13 phage library, which was donated to us from another lab. It yields binders that we have since validated, so there's no issue with the library...
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Can anyone help with why my PCR worked one day and not the next? I am attempting to PCR amplify my empty vector backbone ahead of Gibson Assembly and am using one backbone with two sets of...
30 March 2021 6,131 2 View