Hey everyone,
we would like to grow cells of one cell line in a way that it sticks to the top of cells of another cell line like it is depicted schematically in the attached file. The easiest way would probably be to just add cell on top of a confluent layer, but we would like to avoid that. Instead, we would like to have single cells of one line with cells of the other cell line attached mainly not to the coverslip, but to the top of the first cell line.
Does anybody have a method how to do that?
Thanks,
Leonhard
I think, in general it would be very challenging to achieve. Since the cells are usually motile, the upper will probably sink down to the surface of coverslip just because of gravity. One possibility to avoid this would be to attach the first cell line to the coverslips at desired density, add the second cell line, invert the coverslip and hope that some of the second cells will attach to the first one.
Hi Leonhard,
Here is my theory,
Use the attached cells for the first line and grow them. Then add suspension cells for the second line. Overexpress Cadherin protein in suspension cells.
Good luck
Hello
The book and discussion on RG link will help you
Good Luck
https://www.researchgate.net/post/What_can_I_do_to_make_LNCaP_cells_grow_in_a_monolayer_rather_than_in_aggregates
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