How to grow cells on top of each other?

Hey everyone, we would like to grow cells of one cell line in a way that it sticks to the top of cells of another cell line like it is depicted schematically in the attached file. The easiest way...

01 February 2017 7,533 3 View

How to crop large tif-stacks?

Hey everyone, I need to crop a stack of single tif-files. To be more specific: I have, for example, a stack of 5000 512x512 tif images where I only need the regions from x=159 to 295 and y=279 to...

31 December 2016 4,325 7 View

How can I verify a decrease in endocytosis by Western-Blot investigation of a Pathway?

Hi everybody, in a recent experiment I could show that upon a certain stimulus the endocytosis of cultivated cells decreases drastically. I did this using fluorescently labeled dextrans, but I...

06 July 2015 2,816 3 View

How can I attach suspension cells to glass slides for fluorescence microscopy investigation?

Hi everyone, I would like to attach MOLM13-cells for fluorescence microscopy. However, they are cultured in suspension and hardly attach to glass, Poly-L-Lysine-coated glass, or the...

01 February 2015 8,884 12 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

How Download/Extract citations of 1 given year ALL my publications in Google Scholar?

If you google this you find a lot of smart guys explaining you how to download 1 year of citations of 1 single paper of yours (or anyone else). I mean 1 year of ALL my papers. or ... ALL years or...

01 March 2021 1,121 9 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

Is there a fast method matching citations to bibliography (manually typed references)?

I need to be able to match in-text citations to a reference list and back again in very large documents (100+ pages) WITHOUT the use of referencing software like Endnote. my method is to type the...

27 February 2021 9,848 1 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

What is the most important parameter of a research work, the impact factor or the h index?

The impact factor is a measure of the importance of a journal based on the number of citations received in a year. ... Articles published in a journal with a high impact factor do not all receive...

24 February 2021 7,481 2 View