I have mollusk extract which extracted by acetone and n-hexane(1:3). I have to give 100, 200 and 300 mg/kg PO. to rat. how to covert drug semi solid to liquid ?or how to give the extract to animal? Kindly suggest me.
dissolve your extract in CMC (Carboxy methyl cellulose) , and give the dose using appropriate feeding needle size ...
suspend your extracted in a suitable (corn) oil, or dissolve in water if possible at a known concentration, say 30mg/ml. Now, having established the LD50 from literature or by conducting an LD50 determination with a set of rats, then decide the dose to administer according to body weights of the rats (e.g 500mg/kg). Since 1kg is 1000g,
dose "500mg" = 1000g
"Xmg" = weight of rat (e.g 120g).....find "Xmg" (i.e., 60mg for rat weighing 120g, 50mg for 100g rat)
Then;
Stock 600mg=10ml (i.e, 30mg dissolved in 1ml dH2O, buffer or suspended in suitable oil)
"60mg" will be contained in 1ml, 50mg will be contained in 0.83ml (It is better to have maximum volume to be administered not to exceed 1ml).
You then administer using oral gavage/canular (needing easy-to-learn animal handling training).
Hope this will be helpful.
Cheers!
Hi my friend
Oral administration (per os,p.o.)
The simplest method for administration is to give the substance with food or drinking water. However, this is not practicable with those that are unpalatable, insoluble or chemically unstable in drinking water or when they irritate the mucosa of the gastrointestinal tract (Nebendahl, 2000). The daily food and water intake of mouse should be known before the experiment, to calculate the quantity of substance to be mixed (see Part 5 on Animal Husbandry and Production).
Because food and water wastage happens all the time, it is difficult to determine the precise amount of food and water intake and therefore the precise intake of the substance. The only way this can be done is by keeping mice in metabolic cages and recording the wastage.
Intragastric administration
Direct administration by oral gavage is preferred to mixing substances with food or drinking water because the intake of the substances is precisely measured. A ball tip needle is used to prevent damaging the oesophagus and from passing through the glottal opening into the trachea (Figure 32.8). A 22 G ball tip needle is suitable for administration to adult mice and can be obtained from Popper and Sons, Inc. (New Hyde Park, USA). The conscious mouse is manually restrained firmly by gripping a fold of skin from the scruff of neck down the back (Figure 32.9a), immobilization of the head is essential for this procedure (Figure 32.9b).
When the neck is extended the position is vertical. A straight line is formed between the mouth and the cardiac sphincter through the oesophageal orifice (Figure 32.9b). The needle is passed gently through the mouth and pharynx into the oesophagus (Figure 32.9c).
The mouse usually swallows as the feeding needle approaches the pharynx, these swallowing movements can help so that the probe slips through the oesophageal opening. The substance is then administered slowly. If any obstruction is felt, if the mouse coughs, chokes or begins to struggle vigorously after the gavage begins, or if fluid is seen coming out through the nose, these may indicate that the needle has entered the lungs. Any of these signs would necessitate immediate withdrawal of the needle, and the mouse must be observed very carefully. If there is any sign that fluid has got into the lungs, the mouse should be euthanized. As soon as administration is finished, the needle must be withdrawn (Cunliffe-Beamer and Les, 1987; Suckow et al., 2000). A volume of 2 ml is recommended.
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