01 January 1970 4 6K Report

I would like to purchase a general GFP reporter plasmid from Addgene but it is not available for the profit organization. Could anyone please give me advice how to deal with this problem?

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How to Genotyping of point mutation mouse?

Hi guys, We introduce some mice with two adjacent point mutation of specific molecules, however, it is difficult to carry out genotyping of this mice. DNA base pair and protein size is similar...

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When doing the ADP-Glo kinase assay, will the incubation time affect the experimental results?

Hello, I performed the ADP-Glo kinase assay. In that case, samples(active protein+substrate+ATP+Kinase buffer+w/wo test compound mixture) were incubated for 45 minutes in 30℃. In the next...

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Peek fitting to sus ?

Hi, For some technical question. I have old HPLC machine, For HPLC, detector was died, so I'm going to use it for dual head general pump not for analytics. This machine pump's out line...

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Water uptake isotherm P/Po = RH ?

Hi, I have a question about isotherm. I'm recently researching MOF especially water sorption. I use ASAP 3 Flex, and the result is P/Po as x-axis. Is it same meaning of RH% ? (relative...

07 June 2023 3,007 0 View

Can you teach me about polycistronic vector cloning and overlap PCP using 2A sequence?

Hello, I'd like to ask for your help because polycystronic vector cloning is not working well using 2A sequence. The final product we want to make is as follows restriction enzyme1 - Target1 -...

21 May 2023 6,328 2 View

Why does my FRF oscillate?

Hello. I have question about the FRF result. I performed the vibration test and got some FRF data. As can be seen the following figure, the FRF result is oscillated from 70Hz to 75Hz. This...

20 May 2023 2,243 3 View

How to do DIp Coating for MOF ?

Hello, I would like to do dip coating for MOF material on fiber, cellulose and so on. MOF is kind of very fine power, and It was very difficult to do coating. Could you advise ? and Any coating...

26 April 2023 9,209 1 View

What is the formula for the change in viscosity with concentration?

Given the viscosity of a solution of a known concentration, can the change in viscosity when the concentration changes be calculated by a formula?

02 April 2023 6,816 5 View

What are some commonly used staining reagents for staining the cell membrane of endothelial cells?

Dear community, Recently, I'm doing an experiment to measure the permeability of endothelial cells(HUVEC, HPAEC). I understand that it is important to make sure that endothelial cells form...

14 March 2023 7,689 2 View

Can I use a pan phosphatase inhibitor directly into a cell culture to treat cells before treating cells with a DNA damaging agent like UV?

Thank you for taking time to read this. in a cell culture plate with confluent cells, I'd like to treat them with a pan phosphatase inhibitor and then subsequently treat them with DNA damaging...

09 November 2022 6,806 3 View

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Transfection in HEK293T cells?

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