Where can I buy Agilent software?

Hello. I have old agilent instrument, 6340 with 1100series HPLC, LC-Ion trap MS. We have software for LC, chemstation, but, not for MS. I asked Agilent korea if I can buy some software, and they...

05 January 2021 1,970 3 View

I put a sodium chloride which is less than solubility chart but it doesn't melt. can you tell me what the problem is?

24 September 2020 224 3 View

Does anyone has a cif file for Pt-Ni alloy metal?

Hello. I am trying to fit the EXAFS data of Pt-Ni (around 1:1) alloy nano particles on oxide support. - How can i fit the Pt-Ni metallic peak which is a bit different from Ni metal and Pt metal...

21 July 2020 8,044 3 View

How to check the existence of pinholes in ZnO thin films?

I fabricated a devices whose structure is "bottom electrode(ITO thin film sputterted)" - ZnO thin film - "top electrode(Ag thin film)". The thickness of ZnO thin film is about 200nm. The ZnO thin...

10 March 2019 9,591 3 View

Any available stimuli set for (dynamic) genuine vs. posed emotions?

Hello, I'm designing an experiment that uses a stimuli set that captures and contrasts posed (fake) vs. genuine (authentic) facial expressions. I'm specifically targeting morally relevant...

30 July 2018 2,056 3 View

Is it possible to use part unit of HPLC/MSD?

Our lab has used the LC/MS(LCQ-Deca XP,Thermo, it is still working condition and not walking with PC). LC(Shiseido) is working as stand alone now. Recently, it disconnect to MSD. MSD is not...

19 February 2018 8,547 3 View

Is it ok that the Al thin film is transparent?

Hello. I'm curious about the transparency of Al thin film with sputtering method. I made Aluminium(Al) thin film with sputtering method which has ~ 10nm thickness on a transparent substrate....

18 December 2017 9,651 11 View

Why is nanowire better than thin film in piezoelectric nanogenerator?

19 August 2017 6,980 1 View

Should two fluorescent dyes contact for happening FRET?

18 April 2017 1,644 2 View

Is it possible to use Prep HPLC as casual HPLC?

25 May 2016 3,213 7 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What are the application of Fixed points thoery in Chemistry.?

It is said that fixed point theory has lot of applications not in the field of mathematics only but also in various areas. So I am looking for applications in Chemistry only..

26 February 2021 3,533 1 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View